Endocrine Abstracts

Agouti related protein (AGRP) as a endogenous antagonist of melanocortin 4 receptor plays an important role in regulation of food intake and energy balance being one of the most potent orexigenic factors. We have determined complete sequence of AGRP gene and upstream promoter region in 100 patients with severe obesity (BMI>35). Three previously described polymorphisms were identified: silent mutation G538A in second exon, non-synonymous mutation G772A (rs5030980) and C662T...

Metformin is a biguanide class agent widely used as a first-line treatment for type 2 diabetes, however the detailed mode of action and exact pharmacodynamics has not been clarified. Moreover there is no studies in humans displaying the effect of metformin on DNA methylation. We therefore performed a clinical study involving twelve healthy nondiabetic individuals who were subjected to the 1 week of metformin (2×850 mg/day) treatment. Peripheral blood for DNA isolation was...

Clinical response to metformin is highly variable in type 2 diabetes (T2D) patients highlighting the need for identification of genetic components affecting the efficiency of metformin therapy. Aim of this study was to evaluate the role of systematically selected tagSNPs from genomic regions coding for six organic cation transporters implicated in transport of metformin with respect to the short-term efficiency. The study involved 102 drug naïve newly diagnosed T2D patien...

Association studies between genetic variants of adiponectin gene and susceptibility to type 2 diabetes (T2D) and increased body mass index (BMI) have provided contradictory results. We selected 10 SNPs (rs2241767, rs1501299, rs3777261, rs16861210, rs2241766, rs822396, rs182052, rs17300539, rs16861194, rs266729) in promoter and coding regions of adiponectin gene based on haploblock structure and previously reported association studies. Selected SNPs were screened in 170 patient...